Protein_Domain

Part:BBa_K2401012:Design

Designed by: Shanti Ricke, Alina Nicolai   Group: iGEM17_Hamburg   (2017-10-21)


HMWP 1 High molecular weight synthase 1 expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal NgoMIV site found at 3055
    Illegal NgoMIV site found at 3794
    Illegal NgoMIV site found at 4301
    Illegal NgoMIV site found at 9281
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2467
    Illegal AgeI site found at 3425
    Illegal AgeI site found at 4342
    Illegal AgeI site found at 9581
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2006
    Illegal BsaI.rc site found at 10525
    Illegal SapI site found at 961
    Illegal SapI site found at 6778
    Illegal SapI.rc site found at 1406


Design Notes

As part of our design we distributed HMWP1 and 2 on two different plasmids with different promoter regions so that we can design one expression strain that can theoretically express both our target siderophores with a single set of genes.

Based on the native sequence we had to introduce many point mutations to make sure that the gene is RFC10 compatible.


Source

Genomic sequence Klebsiella pneumonia

References